Consulta: creatorFacets:"Cavelier, Patricia"
Registros recuperados: 3
Data/hora: 19/05/2024 10:34:24

Adult somatic progenitor cells and hematopoiesis in oysters
Provedor de dados: 5
Autores: Jemaa, Mohamed; Morin, Nathalie; Cavelier, Patricia; Cau, Julien; Strub, Jean Marc; Delsert, Claude.
Long-lived animals show a non-observable age-related decline in immune defense, which is provided by blood cells that derive from self-renewing stem cells. The oldest living animals are bivalves. Yet, the origin of hemocytes, the cells involved in innate immunity, is unknown in bivalves and current knowledge about mollusk adult somatic stem cells is scarce. Here we identify a population of adult somatic precursor cells and show their differentiation into hemocytes. Oyster gill contains an as yet unreported irregularly folded structure (IFS) with stem-like cells bathing into the hemolymph. BrdU labeling revealed that the stem-like cells in the gill epithelium and in the nearby hemolymph replicate DNA. Proliferation of this cell population was further...
Tipo: Text
Palavras-chave: Hematopoiesis; Adult somatic progenitor cells; Hemocytes; Mollusk; Marine invertebrates.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00197/30802/29172.pdf

Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers
Provedor de dados: 5
Autores: Cavelier, Patricia; Cau, Julien; Morin, Nathalie; Delsert, Claude.
While our knowledge of bivalve gametogenesis has progressed in recent times, more molecular markers are needed in order to develop tissue imaging. Here, we identified stem cell and mitotic markers to further characterize oyster early gametogenesis, mainly through immunofluorescence microscopy. Intense alkaline phosphatase activity, a non-specific marker for stem cells, was detected on the outer edge of the gonad ducts at the post-spawning stage, suggesting an abundance of undifferentiated cells very early during the sexual cycle. This observation was confirmed using an antibody against Sox2, a transcription factor specific for stem or germline cells, which labeled cells in the gonad duct inner mass and ciliated epithelium early during the initial oyster...
Tipo: Text
Palavras-chave: Germline cells; Cell cycle; Reproduction; Bivalve; Marine invertebrates.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00409/52100/52809.pdf

Microtubule polyglutamylation and acetylation drive microtubule dynamics critical for platelet formation
Provedor de dados: 5
Autores: Van Dijk, Juliette; Bompard, Guillaume; Cau, Julien; Kunishima, Shinji; Rabeharivelo, Gabriel; Mateos-langerak, Julio; Cazevieille, Chantal; Cavelier, Patricia; Boizet-bonhoure, Brigitte; Delsert, Claude; Morin, Nathalie.
Background Upon maturation in the bone marrow, polyploid megakaryocytes elongate very long and thin cytoplasmic branches called proplatelets. Proplatelets enter the sinusoids blood vessels in which platelets are ultimately released. Microtubule dynamics, bundling, sliding, and coiling, drive these dramatic morphological changes whose regulation remains poorly understood. Microtubule properties are defined by tubulin isotype composition and post-translational modification patterns. It remains unknown whether microtubule post-translational modifications occur in proplatelets and if so, whether they contribute to platelet formation. Results Here, we show that in proplatelets from mouse megakaryocytes, microtubules are both acetylated and polyglutamylated. To...
Tipo: Text
Palavras-chave: Microtubules; Acetylation; Polyglutamylation; Tubulin isotype; Platelets; Megakaryocytes; IIb3 integrin; CHO cells.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00463/57463/59577.pdf